| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200215 |
| Chromosome: | chromosome 1 |
| Location: | 6774321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048800 | NAT8 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCACACTGAAAAAATGTTCCGCGCATA |
| Internal bar code: | TGGGACATGTCTTAGTGCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 98.3992 |
| LEAP-Seq n confirming: | 2090 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAACCCTGCTGAACCAC |
| Suggested primer 2: | CTTGTGGTACTTGGCAGCAA |