Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.200228 |
Chromosome: | chromosome_3 |
Location: | 4752449 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g178100 | Lipoxygenase | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGCGCCGCGCTCAAAGTTGTTGGCTGCAA |
Internal bar code: | GCAATTAAGAGGCTCTTGCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 320 |
LEAP-Seq percent confirming: | 99.8404 |
LEAP-Seq n confirming: | 1877 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGTGACGCACATGAACAC |
Suggested primer 2: | CAGCTCGATGCTGATGTTGT |