| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200232 |
| Chromosome: | chromosome 9 |
| Location: | 6624846 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408600 | (1 of 1) 1.13.11.29 - Stizolobate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTTCTCGTCATGCAGCCAATCATCAAA |
| Internal bar code: | TATCTCCTGCGTTGGCGTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 65 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACGCTTAAGAAACGGCT |
| Suggested primer 2: | CACCCATGTACCCTCCATTC |