| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200258 |
| Chromosome: | chromosome 9 |
| Location: | 455575 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404400 | Sin3 complex component; (1 of 1) K14324 - histone deacetylase complex subunit SAP18 (SAP18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGGTAGCCAGGAGGAGGTGCAAACGCT |
| Internal bar code: | GGGGAGCCCCGACTTAAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.5433 |
| LEAP-Seq n confirming: | 7192 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTCATCGAGGGAGATA |
| Suggested primer 2: | GCTTAGTGCCTCCTGACCTG |