| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200268 |
| Chromosome: | chromosome 1 |
| Location: | 3859567 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025000 | SSA12 | cilia-sensing, structure and/or assembly; (1 of 1) K16056 - calcium release-activated calcium channel protein 1 (ORAI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAATGGCAGAAAAAAGTTGTTGTCGAGG |
| Internal bar code: | TTGCTGCAAACTGAAGGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.3839 |
| LEAP-Seq n confirming: | 1613 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAGTATGCTGCAAGTGA |
| Suggested primer 2: | TGAGTGGAGTATCACGAGCG |