| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200301 |
| Chromosome: | chromosome 17 |
| Location: | 4158820 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCGGCTGCGCCTCGTGAAGGTGTGGG |
| Internal bar code: | GCCGCTCATCAATTAGGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 99.7009 |
| LEAP-Seq n confirming: | 4667 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACACTACGCAGACATGG |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |