| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200305 |
| Chromosome: | chromosome 6 |
| Location: | 6882834 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295600 | RME1 | RNA methylase; (1 of 1) PTHR12829//PTHR12829:SF2 - N6-ADENOSINE-METHYLTRANSFERASE // N6-ADENOSINE-METHYLTRANSFERASE 70 KDA SUBUNIT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGGGAGGCAGAGGGATTCCGTGCATA |
| Internal bar code: | ACTGAATTTGCGACTTGCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCACTGGTTTTGAAGGT |
| Suggested primer 2: | GAGTCGCCACAAGGAGTAGC |