| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200316 |
| Chromosome: | chromosome 3 |
| Location: | 3192498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165350 | (1 of 3) 1.1.1.30 - 3-hydroxybutyrate dehydrogenase / D-beta-hydroxybutyrate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCTGTGTTTGCGCCATTGCAAGACATG |
| Internal bar code: | TCGGGATACGCCTAGCGGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 99.3701 |
| LEAP-Seq n confirming: | 6784 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGAGGTTCACCGTATTG |
| Suggested primer 2: | GAGGAGGAGGATAGATGGGC |