Insertion junction: LMJ.RY0402.200342_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.200342
Chromosome:chromosome 1
Location:4770973
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g033000 FAP334 (1 of 2) IPR000157//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Armadillo-type fold; Flagellar Associated Protein 334 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACAGTGATGAACCGACGCACGTGTAAGT
Internal bar code:TTGTATCTGGTGCCCTATGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:99.2908
LEAP-Seq n confirming:140
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCGCACGCTGTAAAGT
Suggested primer 2:CCCCCACTTCTCTGTACCAA