Insertion junction: LMJ.RY0402.200345_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.200345
Chromosome:chromosome 16
Location:4726283
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g686900 (1 of 1) K13120 - protein FAM32A (FAM32A) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCGCCTCCTTCCGCCTCCAACCGCCCC
Internal bar code:ATACGCTTCTAATGAGGGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:44
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:111
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTCACGGTAAGCGAACAT
Suggested primer 2:GTTGCTGCTGTCAAGGATGA