| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200347 |
| Chromosome: | chromosome 16 |
| Location: | 3144557 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665850 | FAP248 | Flagellar Associated Protein 248 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTTGGCACCAGGCCCCCATCGCAAAGC |
| Internal bar code: | GCAGGGCTCCTTTTAGTTCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 99.6508 |
| LEAP-Seq n confirming: | 7419 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGACAGCCTAATACAGCC |
| Suggested primer 2: | GAAAAGCGGCAAACTCAAAG |