| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200358 |
| Chromosome: | chromosome 2 |
| Location: | 4712545 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101900 | EXN6 | (1 of 7) IPR002562//IPR012337 - 3'-5' exonuclease domain // Ribonuclease H-like domain; 3'-5' exonuclease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGTTACCTCGCTACCCTTGCCTAAGCC |
| Internal bar code: | ACCCGGGCACGACCGGGTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 99.5275 |
| LEAP-Seq n confirming: | 1685 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAACCCCGACAACCTCAT |
| Suggested primer 2: | GGTGGAGTTGAAGGCTCTGA |