Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.200367 |
Chromosome: | chromosome_11 |
Location: | 329391 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTTGGCCTTCACGTTCTCGATGGTGTCCG |
Internal bar code: | GCAATGTCTTCGAGCGGCTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1540 |
LEAP-Seq percent confirming: | 82.7586 |
LEAP-Seq n confirming: | 288 |
LEAP-Seq n nonconfirming: | 60 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCCTCCTTGTCCTGGATCT |
Suggested primer 2: | ACCATTGAGAACGTGAAGGC |