| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200368 |
| Chromosome: | chromosome 6 |
| Location: | 2494590 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTGCTCCAGCCGGCCAATGGTGCCGCC |
| Internal bar code: | GAGTCCGGGAGGGACGGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 99.0476 |
| LEAP-Seq n confirming: | 208 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGACTGTTGCGTCTGT |
| Suggested primer 2: | TTTATGTCGCTGGCTCACAG |