Insertion junction: LMJ.RY0402.200380_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.200380
Chromosome:chromosome 4
Location:2857996
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCGCGAGGATACGTTCTTCACGCGCCTG
Internal bar code:ACGCAAGCGTCTCGTGTGTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:993
LEAP-Seq percent confirming:99.8003
LEAP-Seq n confirming:4998
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAGGGGCGTCAGTTTGAA
Suggested primer 2:CGTGAATACAATGCGTGGTC