| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200399 |
| Chromosome: | chromosome 10 |
| Location: | 6352012 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465500 | (1 of 1) 3.1.1.10 - Tropinesterase / Atropinesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCACACACACGCCCCTGCCGCACACGCC |
| Internal bar code: | TGGTCGATGATTGCATACAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 93.4828 |
| LEAP-Seq n confirming: | 13512 |
| LEAP-Seq n nonconfirming: | 942 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAATCCCGCCCACTAAGTA |
| Suggested primer 2: | CGCGTGTGACTGTGTGTATG |