| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200450 |
| Chromosome: | chromosome 6 |
| Location: | 1927470 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263602 | PEX12 | Peroxin 12; (1 of 1) K13345 - peroxin-12 (PEX12, PAF3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGGTAGGAAGCATTTGGCATGGCGGT |
| Internal bar code: | GAAGAACTGGGACGAATTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 98.7966 |
| LEAP-Seq n confirming: | 821 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTAATTCGCAGCTTGGC |
| Suggested primer 2: | GAGCTTGGTAGGGAGAGGCT |