| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200456 |
| Chromosome: | chromosome 14 |
| Location: | 2548098 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625600 | FHL10 | (1 of 2) PF02010 - REJ domain (REJ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCTGGTCAGGGCGCGGCGTGATGAC |
| Internal bar code: | GAATTCTGTACGCCTCCAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 99.6904 |
| LEAP-Seq n confirming: | 322 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCACGAACCGTAAATGT |
| Suggested primer 2: | ACTGCACCCACGACTATTCC |