| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200479 |
| Chromosome: | chromosome 11 |
| Location: | 2127748 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469400 | HIK4,HKR4,HIK | (1 of 1) IPR001789//IPR011006//IPR013761 - Signal transduction response regulator, receiver domain // CheY-like superfamily // Sterile alpha motif/pointed domain; response regulator of two-component system | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGGCTGCGGCTTCAACCATCCCCTCA |
| Internal bar code: | AGTCGCGCTTTGGTTTCTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1162 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCGAAGAACTAACGTGC |
| Suggested primer 2: | AAAAGCTTAAGGAGCAGGGC |