| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200480 |
| Chromosome: | chromosome 1 |
| Location: | 4050366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026400 | EXS1 | (1 of 1) PF03124 - EXS family (EXS); ERD1 homolog | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCAACGATGAACCAGCATTTTCTAGAGG |
| Internal bar code: | GGCGAAAGTCCCTGATCATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCCGCCATCAAGAAGTC |
| Suggested primer 2: | AGACGTGCGTGTACATAGCG |