| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200515 |
| Chromosome: | chromosome_13 |
| Location: | 3108950 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g584901 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTCCTTGTCCAGAGGCACGTGCATCTTGT |
| Internal bar code: | CCATTGCTGCGCGCTAATCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 99.5135 |
| LEAP-Seq n confirming: | 1841 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATAGGTGGTTCTCCCTCA |
| Suggested primer 2: | TGGTGACGCAGTGTTAGGAG |