| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200530 |
| Chromosome: | chromosome 3 |
| Location: | 1649429 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153050 | RWP1 | (1 of 1) IPR000104//IPR003035 - Antifreeze protein, type I // RWP-RK domain; RWP-RK transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCATGGACGGCGTTTGATTTTCCTGGGC |
| Internal bar code: | GCGCCGACCACGCATGAAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 991 |
| LEAP-Seq percent confirming: | 99.3603 |
| LEAP-Seq n confirming: | 466 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCAGAGACAACAGCAA |
| Suggested primer 2: | CTCTCGTTACACGCTGACCA |