| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200541 |
| Chromosome: | chromosome_2 |
| Location: | 462423 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g076500 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCGGCAGGCGCGATTTGCCATTGCCTGGA |
| Internal bar code: | GAGGCCCGTTGGCATTTCATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 75.7895 |
| LEAP-Seq n confirming: | 504 |
| LEAP-Seq n nonconfirming: | 161 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGACAAGGGAAAGGACA |
| Suggested primer 2: | CACCGTGCTCTACTGCTCAA |