| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200566 |
| Chromosome: | chromosome 16 |
| Location: | 1439239 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652400 | FAL18,FAP183 | (1 of 17) PF13202 - EF hand (EF-hand_5); EF-hand domain Flagellar Associated Protein 183 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTGGCCTGCGAGCGCGTGCGACTGCTG |
| Internal bar code: | GTTATCATGGAGTCGGTAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAACAGCCTTTCAACCC |
| Suggested primer 2: | TCATCCTCGTATCAATGCCA |