| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200572 |
| Chromosome: | chromosome 10 |
| Location: | 793673 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423200 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCTCCCGGATTCTCATTGATTGCGACC |
| Internal bar code: | GAATCAAATAACCGCCGACCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.7345 |
| LEAP-Seq n confirming: | 1127 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGGTGTGGATTCATTCT |
| Suggested primer 2: | GAGGTGAGTCAATTCGAGGC |