| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200590 |
| Chromosome: | chromosome 7 |
| Location: | 5333990 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349850 | (1 of 1) IPR000104//IPR001680//IPR017986//IPR020472 - Antifreeze protein, type I // WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCACGGCAGCCGGACAAAACACACGGCT |
| Internal bar code: | GCACAACACCGCCTCTCTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 99.8113 |
| LEAP-Seq n confirming: | 3702 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCCTTGTTCTTCTTCTTG |
| Suggested primer 2: | TGTGAGCATTGCAGTTAGCC |