| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200610 |
| Chromosome: | chromosome 17 |
| Location: | 959379 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703050 | (1 of 97) PF13650 - Aspartyl protease (Asp_protease_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGTTTGGTGTATGTTGGTGCGGCGCGG |
| Internal bar code: | CCACCCTTTCGGGCGAAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 98.703 |
| LEAP-Seq n confirming: | 761 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAATCCCGCCCACTAAGTA |
| Suggested primer 2: | CACTGCTGCTGCTACTGCTC |