Insertion junction: LMJ.RY0402.200654_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.200654
Chromosome:chromosome 16
Location:113378
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g695400 (1 of 2) PF09733 - VEFS-Box of polycomb protein (VEFS-Box) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCCACCCACCCCTTGCCACCTCCCCT
Internal bar code:GCGCTGGGCAGGTCGAGGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:567
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:225
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCCAATCCCAACATACC
Suggested primer 2:GAGTAGGCGCGATTCCTGTA