| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200672 |
| Chromosome: | chromosome 3 |
| Location: | 5785668 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188750 | HEL21 | (1 of 1) K13177 - ATP-dependent RNA helicase DDX1 [EC:3.6.4.13] (DDX1); DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAATGGGGCTAGCGGGAGGCCAGGGGCC |
| Internal bar code: | CTCGGTTTAGGCCCCTTTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1089 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 730 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGAATAACCCCTGCACT |
| Suggested primer 2: | ACCCCACATACACGGACAAT |