| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200696 |
| Chromosome: | chromosome 13 |
| Location: | 3722882 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589250 | (1 of 4) PTHR16255//PTHR16255:SF4 - UNCHARACTERIZED // SPORULATION PROTEIN RMD8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCCTTACGTTGATCAAGCCCCATCAAA |
| Internal bar code: | ACCCCGGTGCATGAGTGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 99.6729 |
| LEAP-Seq n confirming: | 6704 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCATGAATTCCCACTG |
| Suggested primer 2: | GTGTCCTTTGGGTGGAAAGA |