| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200699 |
| Chromosome: | chromosome 9 |
| Location: | 7254658 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412600 | CYG52 | (1 of 1) PF00072//PF00211 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc); Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGTACAGCCGCAAGCACTCGCGGGAGT |
| Internal bar code: | ACAGCTGGTGGTCGGGAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 72.8 |
| LEAP-Seq n confirming: | 182 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGGGACTGGCTATTACAC |
| Suggested primer 2: | GACATGTTGCCGTGCATTAG |