| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200701 |
| Chromosome: | chromosome 13 |
| Location: | 1916717 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576050 | ZIP2,CrZIP10 | (1 of 1) PTHR11040:SF61 - PROTEIN ZNTA; Zinc/iron transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGACGCGCGCTGTGCACGCACCAGTG |
| Internal bar code: | GGTTTTCCTGTCTGGCGCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 98.895 |
| LEAP-Seq n confirming: | 358 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAACCTAGACCACCCTT |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |