| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200714 |
| Chromosome: | chromosome 9 |
| Location: | 1972474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394850 | TEF24 | LrgB-like protein; (1 of 1) PTHR30249 - PUTATIVE SEROTONIN TRANSPORTER | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTGCCCTACGCCTGGCAGTCGCAACGC |
| Internal bar code: | ACGGAAAGCACGAGAAGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 99.8852 |
| LEAP-Seq n confirming: | 5221 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAAGAGGCGCAACTGGTC |
| Suggested primer 2: | AGGGTGTGGACTTTGGAGTG |