| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200722 |
| Chromosome: | chromosome 7 |
| Location: | 6035655 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354850 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAATTATCAACCCACGCGGCCTCTCCAT |
| Internal bar code: | AAACACCCCGGGGTGTTCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 97.6113 |
| LEAP-Seq n confirming: | 899 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGTAACGCACCTAGCG |
| Suggested primer 2: | AAAGCACAACGGTATCCCAG |