| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200743 |
| Chromosome: | chromosome 9 |
| Location: | 2824243 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388800 | GDH1 | Glutamate dehydrogenase; (1 of 2) 1.4.1.3 - Glutamate dehydrogenase (NAD(P)(+)) / Glutamic dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAAAGCACGCCGCACTGAAGTCCCGTGC |
| Internal bar code: | TCCGCCGAGAGGGACAGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 770 |
| LEAP-Seq percent confirming: | 99.6285 |
| LEAP-Seq n confirming: | 2682 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACCCACGTCTTGTGAT |
| Suggested primer 2: | TCTGCGTGGTGCTCTTTATG |