Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.200750 |
Chromosome: | chromosome 7 |
Location: | 3386232 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g335100 | (1 of 5) IPR011051//IPR013096 - RmlC-like cupin domain // Cupin 2, conserved barrel | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGGGAAAAGCCGAAACGCAGGCGGACA |
Internal bar code: | ATTGAGGCTTTGTAGGCTCGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 350 |
LEAP-Seq percent confirming: | 99.773 |
LEAP-Seq n confirming: | 5715 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAGCGAAAGAGCTCACCGA |
Suggested primer 2: | GGTGCCTTGTTTCTCATGGT |