Insertion junction: LMJ.RY0402.200753_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.200753
Chromosome:chromosome 3
Location:5420574
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g185000 GTR8,BGS2,CALS2 Callose synthase 2; (1 of 2) K11000 - callose synthase [EC:2.4.1.-] Glc b1-3 Glc (CALS) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGCGCTGCCTGCACCTGTACCACTCGCT
Internal bar code:TTGGCGGCTCATACACCGAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:30
LEAP-Seq percent confirming:5.88235
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:1744
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCAAAATGAGGAACCTGC
Suggested primer 2:GGTTGAGGAAGGAGGAGGAC