Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.200753 |
Chromosome: | chromosome 3 |
Location: | 5420574 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g185000 | GTR8,BGS2,CALS2 | Callose synthase 2; (1 of 2) K11000 - callose synthase [EC:2.4.1.-] Glc b1-3 Glc (CALS) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCGCTGCCTGCACCTGTACCACTCGCT |
Internal bar code: | TTGGCGGCTCATACACCGAACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 30 |
LEAP-Seq percent confirming: | 5.88235 |
LEAP-Seq n confirming: | 109 |
LEAP-Seq n nonconfirming: | 1744 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTCAAAATGAGGAACCTGC |
Suggested primer 2: | GGTTGAGGAAGGAGGAGGAC |