| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200799 |
| Chromosome: | chromosome 6 |
| Location: | 261105 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250850 | MSH1 | DNA mismatch repair protein, MutS homolog; (1 of 1) PTHR11361:SF82 - DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGGCCGCCTTGCGCTCCCGCGTCTCC |
| Internal bar code: | GTAAGGAGTGGAACGTAGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 99.4432 |
| LEAP-Seq n confirming: | 4465 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGCACATACCGTAACAG |
| Suggested primer 2: | CCTAGGAAGCTGACTCGGTG |