| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200800 |
| Chromosome: | chromosome 8 |
| Location: | 3606073 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375900 | EIF2A-1,EIF2Aa,EIF2X | (1 of 1) K15026 - translation initiation factor 2A (EIF2A); Eukaryotic translation initiation factor 2 subunit 1, eIF2-alpha subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAATGCAATCTGTGCCGTCCCATGCCG |
| Internal bar code: | CGAGGTCGGAACAAAACGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.0236 |
| LEAP-Seq n confirming: | 5882 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCTTCTGACATGTGCCCC |
| Suggested primer 2: | GCTTCTGCCTGAGTGTAGGG |