Insertion junction: LMJ.RY0402.200805_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.200805
Chromosome:chromosome 1
Location:3093373
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g019200 NOP52,GEX18 RRP1-like protein; (1 of 1) K14849 - ribosomal RNA-processing protein 1 (RRP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCACACATCGGTCTTGTACCCGGTCCTC
Internal bar code:CTGTGGTGCCAACTTTGGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:163
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:253
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGACACCAATGCAGAGTCCC
Suggested primer 2:GGAGCAGCAGCATAAGAAGG