| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200808 |
| Chromosome: | chromosome 13 |
| Location: | 1681908 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574100 | (1 of 6) PTHR10994//PTHR10994:SF72 - RETICULON // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTGGTTGCTGTAGCACCGGTGCATGC |
| Internal bar code: | GGAACTGCGGGTCAGCCGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 99.7454 |
| LEAP-Seq n confirming: | 1959 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCAGGTACCTCCGCAAT |
| Suggested primer 2: | ATGCCTGGTCTCTAGGCTCA |