| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200837 |
| Chromosome: | chromosome 15 |
| Location: | 1027365 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638750 | CLR3,RAP17,CLR20,OPR83 | RAP-domain containing protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCATTCAAGCCGCAGGAGCTGTCCAAC |
| Internal bar code: | ACGATACAGGGAGATCGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGCACCTCCTTAGCACT |
| Suggested primer 2: | GAGCGTGTTTGCACAGTGTT |