| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200862 |
| Chromosome: | chromosome 12 |
| Location: | 3033230 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500715 | (1 of 5) 6.2.1.3 - Long-chain-fatty-acid--CoA ligase / Lignoceroyl-CoA synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCAATCCGGTCCCCTCCAGACACAAA |
| Internal bar code: | TCTCAAAGGTGGGGCCGTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 688 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCATCCCATGATCTGCT |
| Suggested primer 2: | GCTGCTCCTGCTTCTCATCT |