| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200864 |
| Chromosome: | chromosome 17 |
| Location: | 59571 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696600 | FMR1 | (1 of 1) PTHR11632:SF6 - PROTEIN F48E8.3; Fumarate reductase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTGTGCGCGCACTTGCCAGAAAAAGC |
| Internal bar code: | GTCCGGACACGAGATTTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCCCAACTTGCATACC |
| Suggested primer 2: | AATGGACTGGTACCACAGGC |