| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200871 |
| Chromosome: | chromosome 3 |
| Location: | 2784137 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162050 | HDA4 | RPD3/HDA1 type histone deacetylase; (1 of 1) PTHR10625//PTHR10625:SF120 - HISTONE DEACETYLASE // HISTONE DEACETYLASE 15 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCACCCCTAGCCCCATGTCACCGCCGC |
| Internal bar code: | CTTAACTGTGGGTGTCCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 99.2366 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCATGTGTGGCGAAGAAT |
| Suggested primer 2: | CCAGTTCTACTCGGCGCTAC |