| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200887 |
| Chromosome: | chromosome 14 |
| Location: | 3784166 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632501 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCGCACCTGACCACCCCTCGCCGCCTC |
| Internal bar code: | AGGCGAACCCCGATGTACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 98.6577 |
| LEAP-Seq n confirming: | 147 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTGCAGGTACCCTCTC |
| Suggested primer 2: | CCCAGACACACACACACACA |