| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200933 |
| Chromosome: | chromosome 3 |
| Location: | 3322715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166350 | (1 of 1) IPR017849//IPR017850 - Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATCTACCTGCTGGACAGCACCCGGGCG |
| Internal bar code: | GAGCCGGTGGGGAGCTCAGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 99.4792 |
| LEAP-Seq n confirming: | 955 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGCCATTTTCCAAGGT |
| Suggested primer 2: | CAGGCCACCCCTACTATCAA |