| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200957 |
| Chromosome: | chromosome_17 |
| Location: | 2701513 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g717950 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGGTGAATTGAAGTCAGCAGGCACAGTAA |
| Internal bar code: | TGAGGAAAGAGAATGCACCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 70.7865 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGTACCACGGAAGTCAT |
| Suggested primer 2: | GCGCAACAATGTAAGCTGAA |