Insertion junction: LMJ.RY0402.200957_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.200957
Chromosome:chromosome 17
Location:2701528
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACGTATTTGAAGCGCCAGGGAGGCGAAG
Internal bar code:CGGTTGCCCCGTCAAGCCTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:634
LEAP-Seq percent confirming:98.961
LEAP-Seq n confirming:381
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGTACCACGGAAGTCAT
Suggested primer 2:GCGCAACAATGTAAGCTGAA