Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.200972 |
Chromosome: | chromosome 12 |
Location: | 6350035 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g537400 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACCCCTTCATCCTCCAATCCCCGCCCG |
Internal bar code: | CGACGCCGACGAGAATCGGTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 457 |
LEAP-Seq percent confirming: | 99.4548 |
LEAP-Seq n confirming: | 3101 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGGGCGGTATAAGTCACA |
Suggested primer 2: | TTTGCTTTTCTTTTGGTGGG |