Insertion junction: LMJ.RY0402.200972_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.200972
Chromosome:chromosome 12
Location:6350035
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g537400 (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAACCCCTTCATCCTCCAATCCCCGCCCG
Internal bar code:CGACGCCGACGAGAATCGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:457
LEAP-Seq percent confirming:99.4548
LEAP-Seq n confirming:3101
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGGCGGTATAAGTCACA
Suggested primer 2:TTTGCTTTTCTTTTGGTGGG